El Primer Dia
I made a couple skirts to wear with my scrub pants and tops. The outfit will look a bit strange (I'll have to post a picture some time), but at least it'll be appropriate. I wonder how many comments I'll get.
I bought three textbooks this afternoon. Ouch. I could have used that $365 for something else. Actually, I might return one or two (which are for my theriogenology class) if it looks like I can handle the class by using books from the vet school library. The third textbook is for the small animal surgery course — it might be a good reference book to have around, and I anticipate reading a fair amount of it. So I think I'll keep that one.
I did an experiment (conjugation) last week to get my 2 DNA circles (plasmids) into listeria monocytogenes. I streaked a whole bunch of E. coli containing the plasmids onto separate plates, and then streaked listeria over the top of the E. coli. Normally I use relatively small amounts of bacteria and try to keep different types separate, so this went completely against the norm. (It seemed like a microbiologist's form of playing in the mud.) I incubated the plates of bacteria for about 5 hours and then suspended some of the bacteria in media and plated them on selective plates (that would select for Listeria based on streptomycin resistance and my plasmids based on chloramphenical resistance — ideally, anything that grew would be listeria containing my plasmids).
Well, it looks like some of the listeria grew! Now I need to determine for sure if the plasmids integrated with the Listeria genome.
Tomorrow I get to do a surgery on a real live dog (I hope it doesn't die). I better study up on the surgery protocol and my suture techniques this afternoon.
I bought three textbooks this afternoon. Ouch. I could have used that $365 for something else. Actually, I might return one or two (which are for my theriogenology class) if it looks like I can handle the class by using books from the vet school library. The third textbook is for the small animal surgery course — it might be a good reference book to have around, and I anticipate reading a fair amount of it. So I think I'll keep that one.
I did an experiment (conjugation) last week to get my 2 DNA circles (plasmids) into listeria monocytogenes. I streaked a whole bunch of E. coli containing the plasmids onto separate plates, and then streaked listeria over the top of the E. coli. Normally I use relatively small amounts of bacteria and try to keep different types separate, so this went completely against the norm. (It seemed like a microbiologist's form of playing in the mud.) I incubated the plates of bacteria for about 5 hours and then suspended some of the bacteria in media and plated them on selective plates (that would select for Listeria based on streptomycin resistance and my plasmids based on chloramphenical resistance — ideally, anything that grew would be listeria containing my plasmids).
Well, it looks like some of the listeria grew! Now I need to determine for sure if the plasmids integrated with the Listeria genome.
Tomorrow I get to do a surgery on a real live dog (I hope it doesn't die). I better study up on the surgery protocol and my suture techniques this afternoon.
posted by Claire at 14:58
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